Microbes as Biofertilisers: NEET MCQs with Answers

15 free questions on Microbes as Biofertilisers for NEET (UG), starting with previous-year NEET and AIPMT questions. Try each one, then tap Show answer for the answer and a short explanation.

Question 1

Previous-year question
Which of the following pairs of plants are used as green manure in crop fields and in sandy soils? [AIPMT 2003]
  1. Saccharum munja and Lantana camara
  2. Dichanthium annulatum and Azolla nilotica
  3. Crotalaria juncea and Alhagi comelorum
  4. Calotropis procera and Phyllanthus niruri
Show answer
Answer: (C) Crotalaria juncea and Alhagi comelorum
Explanation: Crotalaria juncea (sunn hemp) and Alhagi camelorum are used as green manures in India. Sunn hemp is a fast-growing leguminous plant that fixes nitrogen into the soil and is well adapted to sandy soils, while Alhagi improves the soil by adding organic matter.

Question 2

Previous-year question
A free-living nitrogen-fixing cyanobacterium which can also form a symbiotic association with the water fern Azolla is [AIPMT 2004]
  1. Tolypothrix
  2. Chlorella
  3. Nostoc
  4. Anabaena
Show answer
Answer: (D) Anabaena
Explanation: Anabaena is a free-living nitrogen-fixing cyanobacterium which can also form a symbiotic association with the water fern Azolla, living in the cavities of its leaves.

Question 3

Previous-year question
Which one of the following statements is correct? [AIPMT 2007]
  1. Extensive use of chemical fertilisers may lead to eutrophication of nearby water bodies
  2. Both Azotobacter and Rhizobium fix atmospheric nitrogen in root nodules of plants
  3. Cyanobacteria such as Anabaena and Nostoc are important mobilisers of phosphates and potassium for plant nutrition in soil
  4. At present it is not possible to grow maize without chemical fertilisers
Show answer
Answer: (A) Extensive use of chemical fertilisers may lead to eutrophication of nearby water bodies
Explanation: Eutrophication, the nutrient enrichment of a water body, is caused by run-off from fertilised fields, sub-urban lawns, feed lots and detergent-rich sewage. Azotobacter is free-living while Rhizobium is symbiotic; Anabaena and Nostoc are nitrogen-fixers rather than phosphate mobilisers; and maize can be grown with organic manures and compost.

Question 4

Previous-year question
Which one of the following is NOT used in organic farming? [AIPMT 2010]
  1. Oscillatoria
  2. Snail
  3. Glomus
  4. Earthworm
Show answer
Answer: (B) Snail
Explanation: Snails are not used in organic farming because they damage crops by eating leaves and stems, which reduces yield. Earthworms, Glomus and Oscillatoria are beneficial organisms that improve soil fertility.

Question 5

Previous-year question
The common nitrogen-fixer in paddy fields is [AIPMT 2010]
  1. Rhizobium
  2. Azospirillum
  3. Oscillatoria
  4. Frankia
Show answer
Answer: (B) Azospirillum
Explanation: Azospirillum is an associative nitrogen-fixing bacterium of the root zone of cereals such as rice; it is the common nitrogen fixer and biofertiliser of paddy fields.

Question 6

Previous-year question
The free-living, anaerobic nitrogen-fixer is [AIPMT 2010]
  1. Beijerinckia
  2. Rhodospirillum
  3. Rhizobium
  4. Azotobacter
Show answer
Answer: (B) Rhodospirillum
Explanation: Rhodospirillum is a free-living, anaerobic nitrogen-fixer. Beijerinckia and Azotobacter are free-living but aerobic nitrogen-fixing microbes, and Rhizobium is a symbiotic nitrogen-fixer.

Question 7

Previous-year question
Which one of the following is NOT a biofertiliser? [AIPMT 2011]
  1. Rhizobium
  2. Nostoc
  3. Mycorrhiza
  4. Agrobacterium
Show answer
Answer: (D) Agrobacterium
Explanation: Agrobacterium is not a biofertiliser. It is a Gram-negative bacterium that causes tumours in plants; A. tumefaciens carries the Ti-plasmid and causes crown-gall disease, and it is used as an important tool for gene transfer in genetic engineering.
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Question 8

Previous-year question
An organism used as a biofertiliser for raising the soyabean crop is [AIPMT 2011]
  1. Azospirillum
  2. Rhizobium
  3. Nostoc
  4. Azotobacter
Show answer
Answer: (B) Rhizobium
Explanation: Rhizobium leguminosarum is a symbiotic bacterium found in the root nodules of legumes and carries the nitrogen-fixing nif gene. Since soyabean is a legume, Rhizobium is used as its biofertiliser.

Question 9

Previous-year question
A nitrogen-fixing microbe associated with Azolla in rice fields is [AIPMT 2012]
  1. Spirulina
  2. Anabaena
  3. Frankia
  4. Tolypothrix
Show answer
Answer: (B) Anabaena
Explanation: Anabaena azollae is a free-living nitrogen-fixing cyanobacterium which may also live symbiotically in the leaf cavities of Azolla, a free-floating aquatic fern. Because it possesses nitrogenase it can fix nitrogen, and its growth in paddy fields gives a remarkable increase in yield.

Question 10

Previous-year question
Which one of the following microbes forms a symbiotic association with plants and helps them in their nutrition? [AIPMT 2012, 2011]
  1. Azotobacter
  2. Aspergillus
  3. Glomus
  4. Trichoderma
Show answer
Answer: (C) Glomus
Explanation: Several species of Glomus, including G. aggregatum, are cultured and sold as mycorrhizal inoculants for agricultural soils. Being endomycorrhiza, they help the plants in the absorption of nutrients, especially phosphorus, from the soil.

Question 11

Previous-year question
Select the mismatched pair. [NEET 2017]
  1. Rhodospirillum — Mycorrhiza
  2. Anabaena — Nitrogen fixer
  3. Rhizobium — Alfalfa
  4. Frankia — Alnus
Show answer
Answer: (A) Rhodospirillum — Mycorrhiza
Explanation: Rhodospirillum is a free-living nitrogen-fixing bacterium and has nothing to do with mycorrhiza. Mycorrhiza is a symbiotic association between fungi and roots of higher plants, its commonest fungal partners being Glomus species.

Question 12

Previous-year question
Among the following pairs of microbes, which pair has both the microbes usable as biofertilisers? [NEET (Odisha) 2019]
  1. Aspergillus and Rhizopus
  2. Rhizobium and Rhizopus
  3. Cyanobacteria and Rhizobium
  4. Aspergillus and Cyanobacteria
Show answer
Answer: (C) Cyanobacteria and Rhizobium
Explanation: Biofertilisers are organisms that enrich the nutrient quality of the soil. Cyanobacteria fix atmospheric nitrogen in free-living or symbiotic form and Rhizobium fixes nitrogen in root nodules of legumes; Aspergillus and Rhizopus are not used as biofertilisers.

Question 13

Previous-year question
Which one of the following is NOT used in organic farming? [2008]
  1. Oscillatoria
  2. Glomus
  3. Snail
  4. Earthworm
Show answer
Answer: (C) Snail
Explanation: Snails are garden pests that damage crops rather than aid them. Oscillatoria fixes nitrogen, Glomus improves phosphorus uptake, and earthworms aerate the soil and enrich it with castings, so all three are valued in organic practice.

Question 14

Previous-year question
Which microbe forms a symbiotic association with plants and helps in their nutrition? [2012]
  1. Glomus
  2. Azotobacter
  3. Trichoderma
  4. Aspergillus
Show answer
Answer: (A) Glomus
Explanation: Mycorrhizal fungi of the genus Glomus greatly extend the absorbing surface of the root and supply phosphorus and other minerals and water, receiving sugars in exchange. Plants so colonised also show greater resistance to root pathogens and to drought.

Question 15

Previous-year question
An organism used as a biofertiliser for raising soybean crops is [2012]
  1. Rhizobium
  2. Azotobacter
  3. Nostoc
  4. Azospirillum
Show answer
Answer: (A) Rhizobium
Explanation: Soybean is a legume, and Rhizobium invades its root hairs and forms nodules within which it fixes atmospheric nitrogen, receiving carbohydrate from the host in return. Seed is therefore inoculated with the appropriate strain before sowing.

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